Dynamic Nuclear Polarization of Deuterated Proteins
نویسندگان
چکیده
منابع مشابه
Dynamic nuclear polarization of deuterated proteins.
Magic-angle spinning nuclear magnetic resonance (MAS NMR) has evolved as a robust and widely applicable technique for investigating the structure and dynamics of biological systems . It is in fact rapidly becoming an indispensible tool in structural biology studies of amyloid, [4, 5] nanocrystalline, 7] and membrane proteins . Concurrently, it is clear that the low sensitivity of directly detec...
متن کاملA method for dynamic nuclear polarization enhancement of membrane proteins.
Dynamic nuclear polarization (DNP) magic-angle spinning (MAS) solid-state NMR (ssNMR) spectroscopy has the potential to enhance NMR signals by orders of magnitude and to enable NMR characterization of proteins which are inherently dilute, such as membrane proteins. In this work spin-labeled lipid molecules (SL-lipids), when used as polarizing agents, lead to large and relatively homogeneous DNP...
متن کاملCross-polarization for dissolution dynamic nuclear polarization.
Dynamic nuclear polarization (DNP) in combination with subsequent dissolution of the sample allows the detection of low-γ nuclei in the solution state with a signal gain of up to tens of thousand times compared to experiments starting from Boltzmann conditions. The long polarization build-up times of typically more than one hour are a drawback of this technique. The combination of dissolution D...
متن کاملChemically Induced Dynamic Nuclear Polarization
N M R emission and e n h a n c e d absorpt i on o f p roduc t s o f radical react ions ( C I D N P ) is e x p l a i n e d b y nuc lear spin d e p e n d e n t ad iabat i c transit ions be tween singlet a n d tr iplet states o f transient radical pairs. I n m a g n e t i c fields larger than a b o u t 1 k G o n l y S — To-transit ions c ont r ibute t o nuc lear polar izat ion. F r o m a k inet i ...
متن کاملHigh frequency dynamic nuclear polarization.
During the three decades 1980-2010, magic angle spinning (MAS) NMR developed into the method of choice to examine many chemical, physical, and biological problems. In particular, a variety of dipolar recoupling methods to measure distances and torsion angles can now constrain molecular structures to high resolution. However, applications are often limited by the low sensitivity of the experimen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2010
ISSN: 1433-7851
DOI: 10.1002/anie.201002044